mdm2 inhibitor nutlin 3a (MedChemExpress)
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mdm2 inhibitor nutlin 3a
Mdm2 Inhibitor Nutlin 3a, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 96/100, based on 94 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mdm2+inhibitor+nutlin+3a/Nutlin-3a/pmc12465477-57-18-33
Average 96 stars, based on 94 article reviews
Mdm2 Inhibitor Nutlin 3a, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 96/100, based on 94 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mdm2+inhibitor+nutlin+3a/Nutlin-3a/pmc12465477-57-18-33
Average 96 stars, based on 94 article reviews
mdm2 inhibitor nutlin 3a - by Bioz Stars,
2026-10
96/100 stars
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1) Product Images from "RHBDD1 induces cell cycle arrest in TP53-mutant NSCLC cells by promoting endoplasmic reticulum-associated degradation of p53 and DNA-PKcs"
Article Title: RHBDD1 induces cell cycle arrest in TP53-mutant NSCLC cells by promoting endoplasmic reticulum-associated degradation of p53 and DNA-PKcs
Journal: European Journal of Medical Research
doi: 10.1186/s40001-025-03116-y
Figure Legend Snippet: Effect of RHBDD1 on DNA-PKcs/p53 Signaling. A Validation of transfection in A549 cells by qPCR. B Validation of transfection in A549 cells by western blotting. C Levels of RHBDD1, DNA-PKcs, and p53 proteins in sh-RHBDD1-transfected A549 cells treated with the DNA-PKcs inhibitor STL127705. D Protein levels analyzed using ImageJ. E Validation of transfection in NCI-H596 cells by qPCR. F Validation of transfection in in NCI-H596 cells by western blotting. G Levels of RHBDD1, DNA-PKcs and p53 in sh-RHBDD1 transfected NCI-H596 cells treated with STL127705. H The protein levels were analyzed by ImageJ. I Levels of RHBDD1, DNA-PKcs, and p53 in pcDNA-RHBDD1 transfected NCI-H596 cells co-treated with HBX41108 or Nutlin-3a. J Protein levels were analyzed using ImageJ. Data are shown as mean ± SD. N = 3 for all tests. *p < 0.05, **p < 0.01 by one-way ANOVA with Tukey’s multiple comparisons test
Techniques Used: Biomarker Discovery, Transfection, Western Blot
Figure Legend Snippet: RHBDD1 Regulates the Cell Cycle through DNA-PKcs/p53 Signaling. A Cell cycle analysis of transfected NCI-H596 cells co-treated with STL127705 or Nutlin-3a for 48 h by flow cytometry. B Cell cycle distribution. C Viability of transfected NCI-H596 cells co-treated with STL127705 or Nutlin-3a for 48 h. Data are shown as mean ± SD. N = 3 for flow cytometry and N = 6 for CCK-8 assays. *p < 0.05, **p < 0.01 by one-way ANOVA with Tukey’s multiple comparisons test
Techniques Used: Cell Cycle Assay, Transfection, Flow Cytometry, CCK-8 Assay
Figure Legend Snippet: RHBDD1 Regulates DNA-PKcs and p53 Levels in an ERAD-Dependent Manner. A ERAD marker analysis in sh-RHBDD1- or pcDNA-RHBDD1-transfected cells treated with STL127705 or Nutlin-3a, assessed by western blotting. B Relative protein levels in sh-RHBDD1 transfected cells. C Apoptosis induced by cisplatin in pcDNA-RHBDD1-transfected A549 and NCI-H596 cells. Data are shown as mean ± SD. N = 3 for western blot analysis. *p < 0.05, **p < 0.01 by one-way ANOVA with Tukey’s multiple comparisons test
Techniques Used: Marker, Transfection, Western Blot
Related Articles
Biomarker Discovery:Article Title: RHBDD1 induces cell cycle arrest in TP53-mutant NSCLC cells by promoting endoplasmic reticulum-associated degradation of p53 and DNA-PKcs Article Snippet: Cells at 70% confluence were transfected with the vectors in Opti-MEM TM medium (31985070, Invitrogen, USA) using PEI reagent (40815ES03, Yeason, Shanghai, China) for 48 h. After transfection, cells were treated with other reagents and collected within 72 h. The shRNAs used were: shRNA 1: 5′-GATCGCCTATGTTATCACCGCATTTCTCGAGAAATGCGGTGATAACATAGGCTTTTTG-3′; shRNA 2: 5′-GATCGCTGGGATTCTTGTTGGACTATCGAGTAGTCCAACAAGAATCCCAGCTTTTTG-3′.the vectors in Opti-MEM TM medium (31985070, Invitrogen, USA) using PEI reagent (40815ES03, Yeason, Shanghai, China) for 48 h. After transfection, cells were treated with other reagents and collected within 72 h. The shRNAs used were: shRNA 1: 5′-GATCGCCTATGTTATCACCGCATTTCTCGAGAAATGCGGTGATAACATAGGCTTTTTG-3′; shRNA 2: 5′-GATCGCTGGGATTCTTGTTGGACTATCGAGTAGTCCAACAAGAATCCCAGCTTTTTG-3′. ... The DNA-PKcs inhibitor STL127705 (3.5 μM, HY-122727, MCE, Shanghai, China), USP7 inhibitor HBX 41108 (424 nM, HY-101666, MCE), Transfection:Article Title: RHBDD1 induces cell cycle arrest in TP53-mutant NSCLC cells by promoting endoplasmic reticulum-associated degradation of p53 and DNA-PKcs Article Snippet: Cells at 70% confluence were transfected with the vectors in Opti-MEM TM medium (31985070, Invitrogen, USA) using PEI reagent (40815ES03, Yeason, Shanghai, China) for 48 h. After transfection, cells were treated with other reagents and collected within 72 h. The shRNAs used were: shRNA 1: 5′-GATCGCCTATGTTATCACCGCATTTCTCGAGAAATGCGGTGATAACATAGGCTTTTTG-3′; shRNA 2: 5′-GATCGCTGGGATTCTTGTTGGACTATCGAGTAGTCCAACAAGAATCCCAGCTTTTTG-3′.the vectors in Opti-MEM TM medium (31985070, Invitrogen, USA) using PEI reagent (40815ES03, Yeason, Shanghai, China) for 48 h. After transfection, cells were treated with other reagents and collected within 72 h. The shRNAs used were: shRNA 1: 5′-GATCGCCTATGTTATCACCGCATTTCTCGAGAAATGCGGTGATAACATAGGCTTTTTG-3′; shRNA 2: 5′-GATCGCTGGGATTCTTGTTGGACTATCGAGTAGTCCAACAAGAATCCCAGCTTTTTG-3′. ... The DNA-PKcs inhibitor STL127705 (3.5 μM, HY-122727, MCE, Shanghai, China), USP7 inhibitor HBX 41108 (424 nM, HY-101666, MCE), Western Blot:Article Title: RHBDD1 induces cell cycle arrest in TP53-mutant NSCLC cells by promoting endoplasmic reticulum-associated degradation of p53 and DNA-PKcs Article Snippet: Cells at 70% confluence were transfected with the vectors in Opti-MEM TM medium (31985070, Invitrogen, USA) using PEI reagent (40815ES03, Yeason, Shanghai, China) for 48 h. After transfection, cells were treated with other reagents and collected within 72 h. The shRNAs used were: shRNA 1: 5′-GATCGCCTATGTTATCACCGCATTTCTCGAGAAATGCGGTGATAACATAGGCTTTTTG-3′; shRNA 2: 5′-GATCGCTGGGATTCTTGTTGGACTATCGAGTAGTCCAACAAGAATCCCAGCTTTTTG-3′.the vectors in Opti-MEM TM medium (31985070, Invitrogen, USA) using PEI reagent (40815ES03, Yeason, Shanghai, China) for 48 h. After transfection, cells were treated with other reagents and collected within 72 h. The shRNAs used were: shRNA 1: 5′-GATCGCCTATGTTATCACCGCATTTCTCGAGAAATGCGGTGATAACATAGGCTTTTTG-3′; shRNA 2: 5′-GATCGCTGGGATTCTTGTTGGACTATCGAGTAGTCCAACAAGAATCCCAGCTTTTTG-3′. ... The DNA-PKcs inhibitor STL127705 (3.5 μM, HY-122727, MCE, Shanghai, China), USP7 inhibitor HBX 41108 (424 nM, HY-101666, MCE), Cell Cycle Assay:Article Title: RHBDD1 induces cell cycle arrest in TP53-mutant NSCLC cells by promoting endoplasmic reticulum-associated degradation of p53 and DNA-PKcs Article Snippet: Cells at 70% confluence were transfected with the vectors in Opti-MEM TM medium (31985070, Invitrogen, USA) using PEI reagent (40815ES03, Yeason, Shanghai, China) for 48 h. After transfection, cells were treated with other reagents and collected within 72 h. The shRNAs used were: shRNA 1: 5′-GATCGCCTATGTTATCACCGCATTTCTCGAGAAATGCGGTGATAACATAGGCTTTTTG-3′; shRNA 2: 5′-GATCGCTGGGATTCTTGTTGGACTATCGAGTAGTCCAACAAGAATCCCAGCTTTTTG-3′.the vectors in Opti-MEM TM medium (31985070, Invitrogen, USA) using PEI reagent (40815ES03, Yeason, Shanghai, China) for 48 h. After transfection, cells were treated with other reagents and collected within 72 h. The shRNAs used were: shRNA 1: 5′-GATCGCCTATGTTATCACCGCATTTCTCGAGAAATGCGGTGATAACATAGGCTTTTTG-3′; shRNA 2: 5′-GATCGCTGGGATTCTTGTTGGACTATCGAGTAGTCCAACAAGAATCCCAGCTTTTTG-3′. ... The DNA-PKcs inhibitor STL127705 (3.5 μM, HY-122727, MCE, Shanghai, China), USP7 inhibitor HBX 41108 (424 nM, HY-101666, MCE), Flow Cytometry:Article Title: RHBDD1 induces cell cycle arrest in TP53-mutant NSCLC cells by promoting endoplasmic reticulum-associated degradation of p53 and DNA-PKcs Article Snippet: Cells at 70% confluence were transfected with the vectors in Opti-MEM TM medium (31985070, Invitrogen, USA) using PEI reagent (40815ES03, Yeason, Shanghai, China) for 48 h. After transfection, cells were treated with other reagents and collected within 72 h. The shRNAs used were: shRNA 1: 5′-GATCGCCTATGTTATCACCGCATTTCTCGAGAAATGCGGTGATAACATAGGCTTTTTG-3′; shRNA 2: 5′-GATCGCTGGGATTCTTGTTGGACTATCGAGTAGTCCAACAAGAATCCCAGCTTTTTG-3′.the vectors in Opti-MEM TM medium (31985070, Invitrogen, USA) using PEI reagent (40815ES03, Yeason, Shanghai, China) for 48 h. After transfection, cells were treated with other reagents and collected within 72 h. The shRNAs used were: shRNA 1: 5′-GATCGCCTATGTTATCACCGCATTTCTCGAGAAATGCGGTGATAACATAGGCTTTTTG-3′; shRNA 2: 5′-GATCGCTGGGATTCTTGTTGGACTATCGAGTAGTCCAACAAGAATCCCAGCTTTTTG-3′. ... The DNA-PKcs inhibitor STL127705 (3.5 μM, HY-122727, MCE, Shanghai, China), USP7 inhibitor HBX 41108 (424 nM, HY-101666, MCE), CCK-8 Assay:Article Title: RHBDD1 induces cell cycle arrest in TP53-mutant NSCLC cells by promoting endoplasmic reticulum-associated degradation of p53 and DNA-PKcs Article Snippet: Cells at 70% confluence were transfected with the vectors in Opti-MEM TM medium (31985070, Invitrogen, USA) using PEI reagent (40815ES03, Yeason, Shanghai, China) for 48 h. After transfection, cells were treated with other reagents and collected within 72 h. The shRNAs used were: shRNA 1: 5′-GATCGCCTATGTTATCACCGCATTTCTCGAGAAATGCGGTGATAACATAGGCTTTTTG-3′; shRNA 2: 5′-GATCGCTGGGATTCTTGTTGGACTATCGAGTAGTCCAACAAGAATCCCAGCTTTTTG-3′.the vectors in Opti-MEM TM medium (31985070, Invitrogen, USA) using PEI reagent (40815ES03, Yeason, Shanghai, China) for 48 h. After transfection, cells were treated with other reagents and collected within 72 h. The shRNAs used were: shRNA 1: 5′-GATCGCCTATGTTATCACCGCATTTCTCGAGAAATGCGGTGATAACATAGGCTTTTTG-3′; shRNA 2: 5′-GATCGCTGGGATTCTTGTTGGACTATCGAGTAGTCCAACAAGAATCCCAGCTTTTTG-3′. ... The DNA-PKcs inhibitor STL127705 (3.5 μM, HY-122727, MCE, Shanghai, China), USP7 inhibitor HBX 41108 (424 nM, HY-101666, MCE), Marker:Article Title: RHBDD1 induces cell cycle arrest in TP53-mutant NSCLC cells by promoting endoplasmic reticulum-associated degradation of p53 and DNA-PKcs Article Snippet: Cells at 70% confluence were transfected with the vectors in Opti-MEM TM medium (31985070, Invitrogen, USA) using PEI reagent (40815ES03, Yeason, Shanghai, China) for 48 h. After transfection, cells were treated with other reagents and collected within 72 h. The shRNAs used were: shRNA 1: 5′-GATCGCCTATGTTATCACCGCATTTCTCGAGAAATGCGGTGATAACATAGGCTTTTTG-3′; shRNA 2: 5′-GATCGCTGGGATTCTTGTTGGACTATCGAGTAGTCCAACAAGAATCCCAGCTTTTTG-3′.the vectors in Opti-MEM TM medium (31985070, Invitrogen, USA) using PEI reagent (40815ES03, Yeason, Shanghai, China) for 48 h. After transfection, cells were treated with other reagents and collected within 72 h. The shRNAs used were: shRNA 1: 5′-GATCGCCTATGTTATCACCGCATTTCTCGAGAAATGCGGTGATAACATAGGCTTTTTG-3′; shRNA 2: 5′-GATCGCTGGGATTCTTGTTGGACTATCGAGTAGTCCAACAAGAATCCCAGCTTTTTG-3′. ... The DNA-PKcs inhibitor STL127705 (3.5 μM, HY-122727, MCE, Shanghai, China), USP7 inhibitor HBX 41108 (424 nM, HY-101666, MCE), |